In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

Mature oak (Quercus spp.) leaves, although abundantly available during the plants’ developmental cycle, are rarely exploited as viable sources of genomic DNA. These leaves are rich in metabolites difficult to remove during standard DNA purification, interfering with downstream molecular genetics app...

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Bibliographic Details
Main Authors: Csengele E. Barta, Bethany Bolander, Steven R. Bilby, Jeremy H. Brown, Reid N. Brown, Alexander M. Duryee, Danielle R. Edelman, Christina E. Gray, Chandler Gossett, Amie G. Haddock, Mackenzie M. Helsel, Alyssa D. Jones, Marissa E. Klingseis, Kalif Leslie, Edward W. Miles, Rachael A. Prawitz
Format: Article
Language:English
Published: MDPI AG 2017-10-01
Series:Plants
Subjects:
DNA extraction
pin oak (Quercus palustris)
leaves
dark adaptation
secondary metabolites
PCR
gene identification
18S rRNA
isoprene synthase (IspS)
Online Access:https://www.mdpi.com/2223-7747/6/4/52

Internet

https://www.mdpi.com/2223-7747/6/4/52

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