Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells

Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells

We sought to develop a cell-based cytotoxicity assay using human hepatocytes, which reflect the effects of drug-metabolizing enzymes on cytotoxicity. In this study, we generated luminescent human hepatoblastoma HepG2 cells using the mouse artificial chromosome vector, in which click beetle luciferas...

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Bibliographic Details
Main Authors: Satoru Iwado, Satoshi Abe, Mitsuo Oshimura, Yasuhiro Kazuki, Yoshihiro Nakajima
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:International Journal of Molecular Sciences
Subjects:
CYP
hepatotoxicity
HepG2 cells
luciferase
mouse artificial chromosome vector
real-time bioluminescence measurement
Online Access:https://www.mdpi.com/1422-0067/22/6/2843

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https://www.mdpi.com/1422-0067/22/6/2843

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