Inhibition of porcine pancreatic α-amylase activity by chlorogenic acid

Inhibition of porcine pancreatic α-amylase activity by chlorogenic acid

The inhibitory mechanism of chlorogenic acid (CHA) against porcine pancreatic α-amylase (PPA) was examined by enzyme kinetic analysis, circular dichroism, fluorescence quenching and molecular docking. As a result, CHA showed a mixed-type inhibitory action on PPA, with the IC50 value of 0.498 ± 0.013...

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Bibliographic Details
Main Authors: Yuxue Zheng, Wenhan Yang, Weixuan Sun, Shiguo Chen, Donghong Liu, Xiangli Kong, Jinhu Tian, Xingqian Ye
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:Journal of Functional Foods
Subjects:
Porcine pancreatic α-amylase
Chlorogenic acid
Enzyme kinetics
Inhibition mechanism
Molecular docking
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464619305110

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http://www.sciencedirect.com/science/article/pii/S1756464619305110

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