A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity
Human selenium-binding protein 1 (SELENBP1) was originally identified as a protein binding selenium, most likely as selenite. SELENBP1 is associated with cellular redox and thiol homeostasis in several respects, including its established role as a methanethiol oxidase that is involved in degradation...
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doaj-81369527dd3f45ca84991cf3cfbad8a72020-11-24T21:57:28ZengElsevierRedox Biology2213-23172020-01-0128A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicityKarl Köhnlein0Nadine Urban1David Guerrero-Gómez2Holger Steinbrenner3Pavel Urbánek4Josephine Priebs5Philipp Koch6Christoph Kaether7Antonio Miranda-Vizuete8Lars-Oliver Klotz9Institute of Nutritional Sciences, Nutrigenomics Section, Friedrich-Schiller-Universität Jena, Jena, Germany; Leibniz Institute on Aging - Fritz Lipmann Institute, Jena, GermanyInstitute of Nutritional Sciences, Nutrigenomics Section, Friedrich-Schiller-Universität Jena, Jena, GermanyInstituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, SpainInstitute of Nutritional Sciences, Nutrigenomics Section, Friedrich-Schiller-Universität Jena, Jena, GermanyInstitute of Nutritional Sciences, Nutrigenomics Section, Friedrich-Schiller-Universität Jena, Jena, GermanyInstitute of Nutritional Sciences, Nutrigenomics Section, Friedrich-Schiller-Universität Jena, Jena, GermanyLeibniz Institute on Aging - Fritz Lipmann Institute, Jena, GermanyLeibniz Institute on Aging - Fritz Lipmann Institute, Jena, GermanyInstituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, SpainInstitute of Nutritional Sciences, Nutrigenomics Section, Friedrich-Schiller-Universität Jena, Jena, Germany; Corresponding author. Institute of Nutritional Sciences, Nutrigenomics Section, Friedrich Schiller University Jena, Dornburger Strasse 29, Jena, D-07743, Germany.Human selenium-binding protein 1 (SELENBP1) was originally identified as a protein binding selenium, most likely as selenite. SELENBP1 is associated with cellular redox and thiol homeostasis in several respects, including its established role as a methanethiol oxidase that is involved in degradation of methanethiol, a methionine catabolite, generating hydrogen sulfide (H2S) and hydrogen peroxide (H2O2). As both H2S and reactive oxygen species (such as H2O2) are major regulators of Caenorhabditis elegans lifespan and stress resistance, we hypothesized that a SELENBP1 ortholog in C. elegans would likely be involved in regulating these aspects.Here we characterize Y37A1B.5, a putative selenium-binding protein 1 ortholog in C. elegans with 52% primary structure identity to human SELENBP1. While conferring resistance to toxic concentrations of selenite, Y37A1B.5 also attenuates resistance to oxidative stress and lowers C. elegans lifespan: knockdown of Y37A1B.5 using RNA interference resulted in an approx. 10% increase of C. elegans lifespan and an enhanced resistance against the redox cycler paraquat, as well as enhanced motility. Analyses of transgenic reporter strains suggest hypodermal expression and cytoplasmic localization of Y37A1B.5, whose expression decreases with worm age. We identify the transcriptional coregulator MDT-15 and transcription factor EGL-27 as regulators of Y37A1B.5 levels and show that the lifespan extending effect elicited by downregulation of Y37A1B.5 is independent of known MDT-15 interacting factors, such as DAF-16 and NHR-49. In summary, Y37A1B.5 is an ortholog of SELENBP1 that shortens C. elegans lifespan and lowers resistance against oxidative stress, while allowing for a better survival under toxic selenite concentrations. Keywords: Selenium-binding protein, Stress signaling, Caenorhabditis elegans, Lifespanhttp://www.sciencedirect.com/science/article/pii/S2213231719308419 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karl Köhnlein Nadine Urban David Guerrero-Gómez Holger Steinbrenner Pavel Urbánek Josephine Priebs Philipp Koch Christoph Kaether Antonio Miranda-Vizuete Lars-Oliver Klotz |
spellingShingle |
Karl Köhnlein Nadine Urban David Guerrero-Gómez Holger Steinbrenner Pavel Urbánek Josephine Priebs Philipp Koch Christoph Kaether Antonio Miranda-Vizuete Lars-Oliver Klotz A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity Redox Biology |
author_facet |
Karl Köhnlein Nadine Urban David Guerrero-Gómez Holger Steinbrenner Pavel Urbánek Josephine Priebs Philipp Koch Christoph Kaether Antonio Miranda-Vizuete Lars-Oliver Klotz |
author_sort |
Karl Köhnlein |
title |
A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity |
title_short |
A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity |
title_full |
A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity |
title_fullStr |
A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity |
title_full_unstemmed |
A Caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity |
title_sort |
caenorhabditis elegans ortholog of human selenium-binding protein 1 is a pro-aging factor protecting against selenite toxicity |
publisher |
Elsevier |
series |
Redox Biology |
issn |
2213-2317 |
publishDate |
2020-01-01 |
description |
Human selenium-binding protein 1 (SELENBP1) was originally identified as a protein binding selenium, most likely as selenite. SELENBP1 is associated with cellular redox and thiol homeostasis in several respects, including its established role as a methanethiol oxidase that is involved in degradation of methanethiol, a methionine catabolite, generating hydrogen sulfide (H2S) and hydrogen peroxide (H2O2). As both H2S and reactive oxygen species (such as H2O2) are major regulators of Caenorhabditis elegans lifespan and stress resistance, we hypothesized that a SELENBP1 ortholog in C. elegans would likely be involved in regulating these aspects.Here we characterize Y37A1B.5, a putative selenium-binding protein 1 ortholog in C. elegans with 52% primary structure identity to human SELENBP1. While conferring resistance to toxic concentrations of selenite, Y37A1B.5 also attenuates resistance to oxidative stress and lowers C. elegans lifespan: knockdown of Y37A1B.5 using RNA interference resulted in an approx. 10% increase of C. elegans lifespan and an enhanced resistance against the redox cycler paraquat, as well as enhanced motility. Analyses of transgenic reporter strains suggest hypodermal expression and cytoplasmic localization of Y37A1B.5, whose expression decreases with worm age. We identify the transcriptional coregulator MDT-15 and transcription factor EGL-27 as regulators of Y37A1B.5 levels and show that the lifespan extending effect elicited by downregulation of Y37A1B.5 is independent of known MDT-15 interacting factors, such as DAF-16 and NHR-49. In summary, Y37A1B.5 is an ortholog of SELENBP1 that shortens C. elegans lifespan and lowers resistance against oxidative stress, while allowing for a better survival under toxic selenite concentrations. Keywords: Selenium-binding protein, Stress signaling, Caenorhabditis elegans, Lifespan |
url |
http://www.sciencedirect.com/science/article/pii/S2213231719308419 |
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