Specificity Re-evaluation of Oligonucleotide Probes for the Detection of Marine Picoplankton by Tyramide Signal Amplification-Fluorescent In Situ Hybridization

Specificity Re-evaluation of Oligonucleotide Probes for the Detection of Marine Picoplankton by Tyramide Signal Amplification-Fluorescent In Situ Hybridization

Oligonucleotide probes are increasingly being used to characterize natural microbial assemblages by Tyramide Signal Amplification-Fluorescent in situ Hybridization (TSA-FISH, or CAtalysed Reporter Deposition CARD-FISH). In view of the fast-growing rRNA databases, we re-evaluated the in silico specif...

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Bibliographic Details
Main Authors: Virginie Riou, Marine Périot, Isabelle C. Biegala
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-05-01
Series:Frontiers in Microbiology
Subjects:
TSA-FISH
probes
specificity
marine
prokaryotes
eukaryotes
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.00854/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.00854/full

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