Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure.
Increased activity of single ventricular L-type Ca(2+)-channels (L-VDCC) is a hallmark in human heart failure. Recent findings suggest differential modulation by several auxiliary beta-subunits as a possible explanation.By molecular and functional analyses of human and murine ventricles, we find tha...
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2007-03-01
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doaj-818264fd5b09409aa45aa7ddc4caf7362020-11-25T01:47:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-03-0123e29210.1371/journal.pone.0000292Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure.Roger HullinJan MatthesSibylle von VietinghoffIlona BodiMarta RubioKaren D'SouzaIsmail Friedrich KhanDennis RottländerUta C HoppePaul MohacsiEva SchmitteckertRalf GilsbachMoritz BünemannLutz HeinArnold SchwartzStefan HerzigIncreased activity of single ventricular L-type Ca(2+)-channels (L-VDCC) is a hallmark in human heart failure. Recent findings suggest differential modulation by several auxiliary beta-subunits as a possible explanation.By molecular and functional analyses of human and murine ventricles, we find that enhanced L-VDCC activity is accompanied by altered expression pattern of auxiliary L-VDCC beta-subunit gene products. In HEK293-cells we show differential modulation of single L-VDCC activity by coexpression of several human cardiac beta-subunits: Unlike beta(1) or beta(3) isoforms, beta(2a) and beta(2b) induce a high-activity channel behavior typical of failing myocytes. In accordance, beta(2)-subunit mRNA and protein are up-regulated in failing human myocardium. In a model of heart failure we find that mice overexpressing the human cardiac Ca(V)1.2 also reveal increased single-channel activity and sarcolemmal beta(2) expression when entering into the maladaptive stage of heart failure. Interestingly, these animals, when still young and non-failing ("Adaptive Phase"), reveal the opposite phenotype, viz: reduced single-channel activity accompanied by lowered beta(2) expression. Additional evidence for the cause-effect relationship between beta(2)-subunit expression and single L-VDCC activity is provided by newly engineered, double-transgenic mice bearing both constitutive Ca(V)1.2 and inducible beta(2) cardiac overexpression. Here in non-failing hearts induction of beta(2)-subunit overexpression mimicked the increase of single L-VDCC activity observed in murine and human chronic heart failure.Our study presents evidence of the pathobiochemical relevance of beta(2)-subunits for the electrophysiological phenotype of cardiac L-VDCC and thus provides an explanation for the single L-VDCC gating observed in human and murine heart failure.http://europepmc.org/articles/PMC1808423?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roger Hullin Jan Matthes Sibylle von Vietinghoff Ilona Bodi Marta Rubio Karen D'Souza Ismail Friedrich Khan Dennis Rottländer Uta C Hoppe Paul Mohacsi Eva Schmitteckert Ralf Gilsbach Moritz Bünemann Lutz Hein Arnold Schwartz Stefan Herzig |
spellingShingle |
Roger Hullin Jan Matthes Sibylle von Vietinghoff Ilona Bodi Marta Rubio Karen D'Souza Ismail Friedrich Khan Dennis Rottländer Uta C Hoppe Paul Mohacsi Eva Schmitteckert Ralf Gilsbach Moritz Bünemann Lutz Hein Arnold Schwartz Stefan Herzig Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure. PLoS ONE |
author_facet |
Roger Hullin Jan Matthes Sibylle von Vietinghoff Ilona Bodi Marta Rubio Karen D'Souza Ismail Friedrich Khan Dennis Rottländer Uta C Hoppe Paul Mohacsi Eva Schmitteckert Ralf Gilsbach Moritz Bünemann Lutz Hein Arnold Schwartz Stefan Herzig |
author_sort |
Roger Hullin |
title |
Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure. |
title_short |
Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure. |
title_full |
Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure. |
title_fullStr |
Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure. |
title_full_unstemmed |
Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure. |
title_sort |
increased expression of the auxiliary beta(2)-subunit of ventricular l-type ca(2)+ channels leads to single-channel activity characteristic of heart failure. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2007-03-01 |
description |
Increased activity of single ventricular L-type Ca(2+)-channels (L-VDCC) is a hallmark in human heart failure. Recent findings suggest differential modulation by several auxiliary beta-subunits as a possible explanation.By molecular and functional analyses of human and murine ventricles, we find that enhanced L-VDCC activity is accompanied by altered expression pattern of auxiliary L-VDCC beta-subunit gene products. In HEK293-cells we show differential modulation of single L-VDCC activity by coexpression of several human cardiac beta-subunits: Unlike beta(1) or beta(3) isoforms, beta(2a) and beta(2b) induce a high-activity channel behavior typical of failing myocytes. In accordance, beta(2)-subunit mRNA and protein are up-regulated in failing human myocardium. In a model of heart failure we find that mice overexpressing the human cardiac Ca(V)1.2 also reveal increased single-channel activity and sarcolemmal beta(2) expression when entering into the maladaptive stage of heart failure. Interestingly, these animals, when still young and non-failing ("Adaptive Phase"), reveal the opposite phenotype, viz: reduced single-channel activity accompanied by lowered beta(2) expression. Additional evidence for the cause-effect relationship between beta(2)-subunit expression and single L-VDCC activity is provided by newly engineered, double-transgenic mice bearing both constitutive Ca(V)1.2 and inducible beta(2) cardiac overexpression. Here in non-failing hearts induction of beta(2)-subunit overexpression mimicked the increase of single L-VDCC activity observed in murine and human chronic heart failure.Our study presents evidence of the pathobiochemical relevance of beta(2)-subunits for the electrophysiological phenotype of cardiac L-VDCC and thus provides an explanation for the single L-VDCC gating observed in human and murine heart failure. |
url |
http://europepmc.org/articles/PMC1808423?pdf=render |
work_keys_str_mv |
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