The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues

The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues

Abstract Background Utilization of RNA sequencing methods to measure gene expression from archival formalin-fixed paraffin-embedded (FFPE) tumor samples in translational research and clinical trials requires reliable interpretation of the impact of pre-analytical variables on the data obtained, part...

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Bibliographic Details
Main Authors: Michal Marczyk, Chunxiao Fu, Rosanna Lau, Lili Du, Alexander J. Trevarton, Bruno V. Sinn, Rebekah E. Gould, Lajos Pusztai, Christos Hatzis, W. Fraser Symmans
Format: Article
Language:English
Published: BMC 2019-12-01
Series:BMC Cancer
Subjects:
RNA extraction
RNA sequencing
FFPE-based clinical assay
Online Access:https://doi.org/10.1186/s12885-019-6363-0

Internet

https://doi.org/10.1186/s12885-019-6363-0

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