MS-H: a novel proteomic approach to isolate and type the E. coli H antigen using membrane filtration and liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Serotyping is the long-standing gold standard method to determine E. coli H antigens; however, this method requires a panel of H-antigen specific antibodies and often culture-based induction of the H-antigen flagellar motility. In this study, a rapid and accurate method to isolate and identify the E...

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Bibliographic Details
Main Authors: Keding Cheng, Mike Drebot, Joanne McCrea, Lorea Peterson, David Lee, Stuart McCorrister, Richard Nickel, Alyssia Gerbasi, Angela Sloan, Debra Janella, Gary Van Domselaar, Daniel Beniac, Tim Booth, Linda Chui, Helen Tabor, Garrett Westmacott, Matthew Gilmour, Gehua Wang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3578835?pdf=render