Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA

Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA

Alphaviruses need to selectively package genomic viral RNA for transmission, but the packaging mechanism remains unclear. Here, Brown et al. combine PAR-CLIP with biotinylated capsid protein (Cp) retrieval and identify multiple Cp binding sites on genomic viral RNA that promote virion formation.

Bibliographic Details
Main Authors: Rebecca S. Brown, Dimitrios G. Anastasakis, Markus Hafner, Margaret Kielian
Format: Article
Language:English
Published: Nature Publishing Group 2020-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-18447-z
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spelling doaj-81af71b108cf49c58d849e413ef24f8d2021-09-19T11:51:51ZengNature Publishing GroupNature Communications2041-17232020-09-0111111610.1038/s41467-020-18447-zMultiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNARebecca S. Brown0Dimitrios G. Anastasakis1Markus Hafner2Margaret Kielian3Department of Cell Biology, Albert Einstein College of MedicineLaboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis and Musculoskeletal and Skin DiseaseLaboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis and Musculoskeletal and Skin DiseaseDepartment of Cell Biology, Albert Einstein College of MedicineAlphaviruses need to selectively package genomic viral RNA for transmission, but the packaging mechanism remains unclear. Here, Brown et al. combine PAR-CLIP with biotinylated capsid protein (Cp) retrieval and identify multiple Cp binding sites on genomic viral RNA that promote virion formation.https://doi.org/10.1038/s41467-020-18447-z
collection DOAJ
language English
format Article
sources DOAJ
author Rebecca S. Brown
Dimitrios G. Anastasakis
Markus Hafner
Margaret Kielian
spellingShingle Rebecca S. Brown
Dimitrios G. Anastasakis
Markus Hafner
Margaret Kielian
Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA
Nature Communications
author_facet Rebecca S. Brown
Dimitrios G. Anastasakis
Markus Hafner
Margaret Kielian
author_sort Rebecca S. Brown
title Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA
title_short Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA
title_full Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA
title_fullStr Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA
title_full_unstemmed Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA
title_sort multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic rna
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-09-01
description Alphaviruses need to selectively package genomic viral RNA for transmission, but the packaging mechanism remains unclear. Here, Brown et al. combine PAR-CLIP with biotinylated capsid protein (Cp) retrieval and identify multiple Cp binding sites on genomic viral RNA that promote virion formation.
url https://doi.org/10.1038/s41467-020-18447-z
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AT dimitriosganastasakis multiplecapsidproteinbindingsitesmediateselectivepackagingofthealphavirusgenomicrna
AT markushafner multiplecapsidproteinbindingsitesmediateselectivepackagingofthealphavirusgenomicrna
AT margaretkielian multiplecapsidproteinbindingsitesmediateselectivepackagingofthealphavirusgenomicrna
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