Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins

Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins

ADP-ribose binding macro domains facilitate the enrichment and detection of cellular ADP-ribosylation. Here, the authors generate an engineered macro domain with increased ADP-ribose affinity, improving the identification of ADP-ribosylated proteins by proteomics, western blot and immunofluorescence...

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Bibliographic Details
Main Authors: Kathrin Nowak, Florian Rosenthal, Tobias Karlberg, Mareike Bütepage, Ann-Gerd Thorsell, Birgit Dreier, Jonas Grossmann, Jens Sobek, Ralph Imhof, Bernhard Lüscher, Herwig Schüler, Andreas Plückthun, Deena M. Leslie Pedrioli, Michael O. Hottiger
Format: Article
Language:English
Published: Nature Publishing Group 2020-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-18981-w

Internet

https://doi.org/10.1038/s41467-020-18981-w

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