Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext)

Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext)

Massively parallel DNA sequencing offers many benefits, but major inhibitory cost factors include: (1) start-up (i.e., purchasing initial reagents and equipment); (2) buy-in (i.e., getting the smallest possible amount of data from a run); and (3) sample preparation. Reducing sample preparation costs...

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Bibliographic Details
Main Authors: Travis C. Glenn, Roger A. Nilsen, Troy J. Kieran, Jon G. Sanders, Natalia J. Bayona-Vásquez, John W. Finger, Todd W. Pierson, Kerin E. Bentley, Sandra L. Hoffberg, Swarnali Louha, Francisco J. Garcia-De Leon, Miguel Angel del Rio Portilla, Kurt D. Reed, Jennifer L. Anderson, Jennifer K. Meece, Samuel E. Aggrey, Romdhane Rekaya, Magdy Alabady, Myriam Belanger, Kevin Winker, Brant C. Faircloth
Format: Article
Language:English
Published: PeerJ Inc. 2019-10-01
Series:PeerJ
Subjects:
Illumina
Next Generation Sequencing
NovaSeq
Sample Preparation
Pooling
Multiplexing
Online Access:https://peerj.com/articles/7755.pdf

Internet

https://peerj.com/articles/7755.pdf

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