Impact of Host DNA and Sequencing Depth on the Taxonomic Resolution of Whole Metagenome Sequencing for Microbiome Analysis

Impact of Host DNA and Sequencing Depth on the Taxonomic Resolution of Whole Metagenome Sequencing for Microbiome Analysis

The amount of host DNA poses a major challenge to metagenome analysis. However, there is no guidance on the levels of host DNA, nor on the depth of sequencing needed to acquire meaningful information from whole metagenome sequencing (WMS). Here, we evaluated the impact of a wide range of amounts of...

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Bibliographic Details
Main Authors: Joana Pereira-Marques, Anne Hout, Rui M. Ferreira, Michiel Weber, Ines Pinto-Ribeiro, Leen-Jan van Doorn, Cornelis Willem Knetsch, Ceu Figueiredo
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-06-01
Series:Frontiers in Microbiology
Subjects:
microbiome analysis
metagenomics
sample complexity
sequencing depth
mock community
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.01277/full

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https://www.frontiersin.org/article/10.3389/fmicb.2019.01277/full

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