APPLICATION OF 6-NITROCOUMARIN AS A SUBSTRATE FOR THE FLUORESCENT DETECTION OF NITROREDUCTASE ACTIVITY IN Sporothrix schenckii

APPLICATION OF 6-NITROCOUMARIN AS A SUBSTRATE FOR THE FLUORESCENT DETECTION OF NITROREDUCTASE ACTIVITY IN Sporothrix schenckii

Introduction Sporothrix schenckii is a thermal dimorphic pathogenic fungus causing a subcutaneous mycosis, sporotrichosis. Nitrocoumarin represents a fluorogenic substrate class where the microbial nitroreductase activity produces several derivatives, already used in several other enzyme assays. The...

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Bibliographic Details
Main Authors: Cheila Denise Ottonelli Stopiglia, Mariana Carissimi, Tatiane Caroline Daboit, Valter Stefani, Valeriano Antonio Corbellini, Maria Lucia Scroferneker
Format: Article
Language:English
Published: Universidade de São Paulo 2013-09-01
Series:Revista do Instituto de Medicina Tropical de São Paulo
Subjects:
Sporotrichosis
Enzymatic activity
Fluorogenic substrate
6-nitrocoumarin
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000500353&lng=en&tlng=en

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