SARS-CoV-2 Direct Detection Without RNA Isolation With Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12

SARS-CoV-2 Direct Detection Without RNA Isolation With Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12

As to date, more than 49 million confirmed cases of Coronavirus Disease 19 (COVID-19) have been reported worldwide. Current diagnostic protocols use qRT-PCR for viral RNA detection, which is expensive and requires sophisticated equipment, trained personnel and previous RNA extraction. For this reaso...

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Bibliographic Details
Main Authors: Alfredo Garcia-Venzor, Bertha Rueda-Zarazua, Eduardo Marquez-Garcia, Vilma Maldonado, Angelica Moncada-Morales, Hiram Olivera, Irma Lopez, Joaquin Zuñiga, Jorge Melendez-Zajgla
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Medicine
Subjects:
COVID-19
SARS-CoV-2
diagnosis
LAMP
CRISPR-Cas12a
Online Access:https://www.frontiersin.org/articles/10.3389/fmed.2021.627679/full

Internet

https://www.frontiersin.org/articles/10.3389/fmed.2021.627679/full

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