Generation of human induced pluripotent stem cells using non-synthetic mRNA

Generation of human induced pluripotent stem cells using non-synthetic mRNA

Here we describe some of the crucial steps to generate induced pluripotent stem cells (iPSCs) using mRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribed mRNA. V. virus' 2′-O-Methyltransferas...

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Bibliographic Details
Main Authors: L. Rohani, C. Fabian, H. Holland, Y. Naaldijk, R. Dressel, H. Löffler-Wirth, H. Binder, A. Arnold, A. Stolzing
Format: Article
Language:English
Published: Elsevier 2016-05-01
Series:Stem Cell Research
Subjects:
Induced pluripotent stem cell
Reprogramming
mRNA
Online Access:http://www.sciencedirect.com/science/article/pii/S187350611630006X

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http://www.sciencedirect.com/science/article/pii/S187350611630006X

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