Generation of human induced pluripotent stem cells using non-synthetic mRNA
Here we describe some of the crucial steps to generate induced pluripotent stem cells (iPSCs) using mRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribed mRNA. V. virus' 2′-O-Methyltransferas...
Main Authors: | L. Rohani, C. Fabian, H. Holland, Y. Naaldijk, R. Dressel, H. Löffler-Wirth, H. Binder, A. Arnold, A. Stolzing |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2016-05-01
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Series: | Stem Cell Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S187350611630006X |
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