Untangling Species-Level Composition of Complex Bacterial Communities through a Novel Metagenomic Approach

Untangling Species-Level Composition of Complex Bacterial Communities through a Novel Metagenomic Approach

We developed a novel method for accurate cataloguing of bacterial communities at (sub)species level involving amplification of the internal transcribed spacer (ITS) region through optimized primers, followed by next-generation sequencing and taxonomic classification of amplicons by means of a compre...

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Bibliographic Details
Main Authors: Christian Milani, Giulia Alessandri, Marta Mangifesta, Leonardo Mancabelli, Gabriele Andrea Lugli, Federico Fontana, Giulia Longhi, Rosaria Anzalone, Alice Viappiani, Sabrina Duranti, Francesca Turroni, Renato Costi, Alfredo Annicchiarico, Andrea Morini, Leopoldo Sarli, Maria Cristina Ossiprandi, Douwe van Sinderen, Marco Ventura
Format: Article
Language:English
Published: American Society for Microbiology 2020-07-01
Series:mSystems
Subjects:
metagenomics
its
profiling
Online Access:https://doi.org/10.1128/mSystems.00404-20

Internet

https://doi.org/10.1128/mSystems.00404-20

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