Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes
Abstract MiR-21, a non-coding miRNA with 22 nucleotides, plays an important part in the proliferation, invasion, and metastasis of tumor cells. The present study demonstrates that isomers of chiral ruthenium(II) complexes with alkynes (Λ-1 and Δ-1) were synthesized by Songogashira coupling reaction...
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doaj-82faed1c6de844caa09e11eb1fb9bb012020-11-25T03:31:58ZengBMCBMC Chemistry2661-801X2020-04-011411710.1186/s13065-020-00672-8Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexesYin Feng0Jing Shu1Liangzhong Yao2Yutao Lan3Lianbao Ye4Wenjie Mei5Ying Ding6The First Affiliated Hospital of Guangdong Pharmaceutical UniversitySchool of Pharmacy, Guangdong Pharmaceutical UniversityThe First Affiliated Hospital of Guangdong Pharmaceutical UniversityGuangdong Province Engineering Center for Molecular Probe & Biomedical ImagingSchool of Pharmacy, Guangdong Pharmaceutical UniversitySchool of Pharmacy, Guangdong Pharmaceutical UniversityThe First Affiliated Hospital of Guangdong Pharmaceutical UniversityAbstract MiR-21, a non-coding miRNA with 22 nucleotides, plays an important part in the proliferation, invasion, and metastasis of tumor cells. The present study demonstrates that isomers of chiral ruthenium(II) complexes with alkynes (Λ-1 and Δ-1) were synthesized by Songogashira coupling reaction by using microwave-assisted synthetic technology. The isomers can recognize and stabilize miR-21, with the Λ-isomer showing a stronger binding capacity than the Δ-isomer. Further studies showed that both isomers can be uptaken by MDA-MB-231 cells and enriched in the nucleus. Treatment with the Λ-/Δ-isomer downregulated the expression of miR-21. In a word, the development of chiral ruthenium(II) complexes act as potential inhibitors against tumor cells by recognizing, stabilizing, and regulating the expression of miR-21.http://link.springer.com/article/10.1186/s13065-020-00672-8Chiral ruthenium(II) complexesMiR-21RNA binding propertyFRET |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yin Feng Jing Shu Liangzhong Yao Yutao Lan Lianbao Ye Wenjie Mei Ying Ding |
spellingShingle |
Yin Feng Jing Shu Liangzhong Yao Yutao Lan Lianbao Ye Wenjie Mei Ying Ding Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes BMC Chemistry Chiral ruthenium(II) complexes MiR-21 RNA binding property FRET |
author_facet |
Yin Feng Jing Shu Liangzhong Yao Yutao Lan Lianbao Ye Wenjie Mei Ying Ding |
author_sort |
Yin Feng |
title |
Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes |
title_short |
Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes |
title_full |
Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes |
title_fullStr |
Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes |
title_full_unstemmed |
Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes |
title_sort |
recognizing and stabilizing mir-21 by chiral ruthenium(ii) complexes |
publisher |
BMC |
series |
BMC Chemistry |
issn |
2661-801X |
publishDate |
2020-04-01 |
description |
Abstract MiR-21, a non-coding miRNA with 22 nucleotides, plays an important part in the proliferation, invasion, and metastasis of tumor cells. The present study demonstrates that isomers of chiral ruthenium(II) complexes with alkynes (Λ-1 and Δ-1) were synthesized by Songogashira coupling reaction by using microwave-assisted synthetic technology. The isomers can recognize and stabilize miR-21, with the Λ-isomer showing a stronger binding capacity than the Δ-isomer. Further studies showed that both isomers can be uptaken by MDA-MB-231 cells and enriched in the nucleus. Treatment with the Λ-/Δ-isomer downregulated the expression of miR-21. In a word, the development of chiral ruthenium(II) complexes act as potential inhibitors against tumor cells by recognizing, stabilizing, and regulating the expression of miR-21. |
topic |
Chiral ruthenium(II) complexes MiR-21 RNA binding property FRET |
url |
http://link.springer.com/article/10.1186/s13065-020-00672-8 |
work_keys_str_mv |
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