A practical synthesis of the 13C/15N-labelled tripeptide N-formyl-Met-Leu-Phe, useful as a reference in solid-state NMR spectroscopy

A practical synthesis of the 13C/15N-labelled tripeptide N-formyl-Met-Leu-Phe, useful as a reference in solid-state NMR spectroscopy

A mild synthetic method for N-formyl-Met-Leu-Phe-OH (1) is described. After Fmoc solid phase peptide synthesis, on-bead formylation and HPLC purification, more than 30 mg of the fully 13C/15N-labelled tripeptide 1 could be isolated in a typical batch. This peptide can be easily crystallised and is t...

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Bibliographic Details
Main Authors: Sven T. Breitung, Jakob J. Lopez, Gerd Dürner, Clemens Glaubitz, Michael W. Göbel, Marcel Suhartono
Format: Article
Language:English
Published: Beilstein-Institut 2008-10-01
Series:Beilstein Journal of Organic Chemistry
Subjects:
Fmoc solid phase peptide synthesis
formylation
f-MLF
magic-angle spinning
Wang resin
Online Access:https://doi.org/10.3762/bjoc.4.35

Internet

https://doi.org/10.3762/bjoc.4.35

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