Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory
<p>Immunomodulatory activity assay and characterization of exopolysaccharide (EPS) from Lactic Acid Bacteria (LAB) was done in Bogor. Bacteria used in this study was LAB strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Exopolysaccharide was extracted from...
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Pusat Penelitian dan Pengembangan Peternakan
2017-09-01
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Online Access: | http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1414 |
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doaj-839eda6478614f909d071335aeb3c74f2020-11-25T00:51:40ZengPusat Penelitian dan Pengembangan PeternakanJurnal Ilmu Ternak dan Veteriner0853-73802252-696X2017-09-0121318218910.14334/jitv.v21i3.14141266Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatoryKusmiati .0Fifi Afiati1Farha Elein Kukihi2Pusat Penelitian Bioteknologi LIPIPusat Penelitian Bioteknologi LIPIInstitut Sain dan Teknologi Nasional<p>Immunomodulatory activity assay and characterization of exopolysaccharide (EPS) from Lactic Acid Bacteria (LAB) was done in Bogor. Bacteria used in this study was LAB strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Exopolysaccharide was extracted from L. delbrueckii subsp. bulgaricus and S. thermophilus then characterized with FT-IR spectrophotometer to determine the functional group. IR spectrum analysis using Fourier Transform-Infra Red (FT-IR) showed that EPS from both LAB isolates were carbohydrate compounds. Immunomodulatory activity in vivo from EPS was measured using phagocytic activity and phagocytic capacity macrophage cells from mice peritoneal cavity fluid. Exopolysaccharide were given orally to mice in concentrations of 100 μg/ml, 200 μg/ml and 300 μg/ml for 14 days then the mice were infected with Staphylococcus aureus. Result showed that EPS from both LAB isolate enhanced either phagocytic activity and phagocytic capacity macrophage cell from mice peritoneal fluid. EPS from L. delbrueckii subsp. bulgaricus concentration 300 μg/ml showed the highest phagocytic activity of macrophage cells and EPS from S. thermophilus concentration 300 μg/ml showed the highest phagocytic capacity. It is concluded that EPS potency tested as immunomodulatory derived from a culture of L. delbrueckii and S. thermophilus subsp.bulgaricus are able to increase the activity and phagocytosis murine peritoneal macrophages.</p>http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1414exopolysaccharidelactic acid bacteria (lab)immunomodulatoryyoghurtfourier transform-infra red (ft-ir) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kusmiati . Fifi Afiati Farha Elein Kukihi |
spellingShingle |
Kusmiati . Fifi Afiati Farha Elein Kukihi Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory Jurnal Ilmu Ternak dan Veteriner exopolysaccharide lactic acid bacteria (lab) immunomodulatory yoghurt fourier transform-infra red (ft-ir) |
author_facet |
Kusmiati . Fifi Afiati Farha Elein Kukihi |
author_sort |
Kusmiati . |
title |
Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory |
title_short |
Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory |
title_full |
Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory |
title_fullStr |
Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory |
title_full_unstemmed |
Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory |
title_sort |
exopolysaccharide (eps) activity test of lactic acid bacteria (lab) as immunomodulatory |
publisher |
Pusat Penelitian dan Pengembangan Peternakan |
series |
Jurnal Ilmu Ternak dan Veteriner |
issn |
0853-7380 2252-696X |
publishDate |
2017-09-01 |
description |
<p>Immunomodulatory activity assay and characterization of exopolysaccharide (EPS) from Lactic Acid Bacteria (LAB) was done in Bogor. Bacteria used in this study was LAB strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Exopolysaccharide was extracted from L. delbrueckii subsp. bulgaricus and S. thermophilus then characterized with FT-IR spectrophotometer to determine the functional group. IR spectrum analysis using Fourier Transform-Infra Red (FT-IR) showed that EPS from both LAB isolates were carbohydrate compounds. Immunomodulatory activity in vivo from EPS was measured using phagocytic activity and phagocytic capacity macrophage cells from mice peritoneal cavity fluid. Exopolysaccharide were given orally to mice in concentrations of 100 μg/ml, 200 μg/ml and 300 μg/ml for 14 days then the mice were infected with Staphylococcus aureus. Result showed that EPS from both LAB isolate enhanced either phagocytic activity and phagocytic capacity macrophage cell from mice peritoneal fluid. EPS from L. delbrueckii subsp. bulgaricus concentration 300 μg/ml showed the highest phagocytic activity of macrophage cells and EPS from S. thermophilus concentration 300 μg/ml showed the highest phagocytic capacity. It is concluded that EPS potency tested as immunomodulatory derived from a culture of L. delbrueckii and S. thermophilus subsp.bulgaricus are able to increase the activity and phagocytosis murine peritoneal macrophages.</p> |
topic |
exopolysaccharide lactic acid bacteria (lab) immunomodulatory yoghurt fourier transform-infra red (ft-ir) |
url |
http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1414 |
work_keys_str_mv |
AT kusmiati exopolysaccharideepsactivitytestoflacticacidbacterialabasimmunomodulatory AT fifiafiati exopolysaccharideepsactivitytestoflacticacidbacterialabasimmunomodulatory AT farhaeleinkukihi exopolysaccharideepsactivitytestoflacticacidbacterialabasimmunomodulatory |
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