Amplification by PCR artificially reduces the proportion of the rare biosphere in microbial communities.

The microbial world has been shown to hold an unimaginable diversity. The use of rRNA genes and PCR amplification to assess microbial community structure and diversity present biases that need to be analyzed in order to understand the risks involved in those estimates. Herein, we show that PCR ampli...

Full description

Bibliographic Details
Main Authors: Juan M Gonzalez, Maria C Portillo, Pedro Belda-Ferre, Alex Mira
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253843/pdf/?tool=EBI