Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus
Extracts of the tropical Cinderella plant Synedrella nodiflora are used traditionally to manage convulsive conditions in the West African sub-region. This study sought to determine the neuronal basis of the effectiveness of these plant extracts to suppress seizure activity. Using the hippocampal sli...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-03-01
|
Series: | Frontiers in Pharmacology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.610025/full |
id |
doaj-83e0485deae346c9a75dda3606a908ec |
---|---|
record_format |
Article |
spelling |
doaj-83e0485deae346c9a75dda3606a908ec2021-03-08T04:25:57ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-03-011210.3389/fphar.2021.610025610025Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat HippocampusPatrick Amoateng0Thomas A. Tagoe1Thomas K. Karikari2Kennedy K. E Kukuia3Dorcas Osei-Safo4Eric Woode5Bruno G. Frenguelli6Samuel B. Kombian7Samuel B. Kombian8Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Accra, GhanaDepartment of Physiology, UG Medical School, College of Health Sciences, University of Ghana, Accra, GhanaDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Medical Pharmacology, UG Medical School, College of Health Sciences, University of Ghana, Accra, GhanaDepartment of Chemistry, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, Accra, GhanaDepartment of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, GhanaSchool of Life Sciences, University of Warwick, Coventry, United KingdomDepartment of Pharmacology and Therapeutics, Faculty of Pharmacy, Health Science Center, Kuwait University, Safat, KuwaitDepartment of Pharmacology and Toxicology, School of Medicine and Medical Sciences, University for Development Studies, Tamale, GhanaExtracts of the tropical Cinderella plant Synedrella nodiflora are used traditionally to manage convulsive conditions in the West African sub-region. This study sought to determine the neuronal basis of the effectiveness of these plant extracts to suppress seizure activity. Using the hippocampal slice preparation from rats, the ability of the extract to depress excitatory synaptic transmission and in vitro seizure activity were investigated. Bath perfusion of the hydro-ethanolic extract of Synedrella nodiflora (SNE) caused a concentration-dependent depression of evoked field excitatory postsynaptic potentials (fEPSPs) recorded extracellularly in the CA1 region of the hippocampus with maximal depression of about 80% and an estimated IC50 of 0.06 mg/ml. The SNE-induced fEPSP depression was accompanied by an increase in paired pulse facilitation. The fEPSP depression only recovered partially after 20 min washing out. The effect of SNE was not stimulus dependent as it was present even in the absence of synaptic stimulation. Furthermore, it did not show desensitization as repeat application after 10 min washout produced the same level of fEPSP depression as the first application. The SNE effect on fEPSPs was not via adenosine release as it was neither blocked nor reversed by 8-CPT, an adenosine A1 receptor antagonist. In addition, SNE depressed in vitro seizures induced by zero Mg2+ and high K+ -containing artificial cerebrospinal fluid (aCSF) in a concentration-dependent manner. The results show that SNE depresses fEPSPs and spontaneous bursting activity in hippocampal neurons that may underlie its ability to abort convulsive activity in persons with epilepsy.https://www.frontiersin.org/articles/10.3389/fphar.2021.610025/fullhippocampal slicesfield excitatory postsynaptic potentialsadenosineSNEseizure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Patrick Amoateng Thomas A. Tagoe Thomas K. Karikari Kennedy K. E Kukuia Dorcas Osei-Safo Eric Woode Bruno G. Frenguelli Samuel B. Kombian Samuel B. Kombian |
spellingShingle |
Patrick Amoateng Thomas A. Tagoe Thomas K. Karikari Kennedy K. E Kukuia Dorcas Osei-Safo Eric Woode Bruno G. Frenguelli Samuel B. Kombian Samuel B. Kombian Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus Frontiers in Pharmacology hippocampal slices field excitatory postsynaptic potentials adenosine SNE seizure |
author_facet |
Patrick Amoateng Thomas A. Tagoe Thomas K. Karikari Kennedy K. E Kukuia Dorcas Osei-Safo Eric Woode Bruno G. Frenguelli Samuel B. Kombian Samuel B. Kombian |
author_sort |
Patrick Amoateng |
title |
Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus |
title_short |
Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus |
title_full |
Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus |
title_fullStr |
Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus |
title_full_unstemmed |
Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus |
title_sort |
synedrella nodiflora extract depresses excitatory synaptic transmission and chemically-induced in vitro seizures in the rat hippocampus |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2021-03-01 |
description |
Extracts of the tropical Cinderella plant Synedrella nodiflora are used traditionally to manage convulsive conditions in the West African sub-region. This study sought to determine the neuronal basis of the effectiveness of these plant extracts to suppress seizure activity. Using the hippocampal slice preparation from rats, the ability of the extract to depress excitatory synaptic transmission and in vitro seizure activity were investigated. Bath perfusion of the hydro-ethanolic extract of Synedrella nodiflora (SNE) caused a concentration-dependent depression of evoked field excitatory postsynaptic potentials (fEPSPs) recorded extracellularly in the CA1 region of the hippocampus with maximal depression of about 80% and an estimated IC50 of 0.06 mg/ml. The SNE-induced fEPSP depression was accompanied by an increase in paired pulse facilitation. The fEPSP depression only recovered partially after 20 min washing out. The effect of SNE was not stimulus dependent as it was present even in the absence of synaptic stimulation. Furthermore, it did not show desensitization as repeat application after 10 min washout produced the same level of fEPSP depression as the first application. The SNE effect on fEPSPs was not via adenosine release as it was neither blocked nor reversed by 8-CPT, an adenosine A1 receptor antagonist. In addition, SNE depressed in vitro seizures induced by zero Mg2+ and high K+ -containing artificial cerebrospinal fluid (aCSF) in a concentration-dependent manner. The results show that SNE depresses fEPSPs and spontaneous bursting activity in hippocampal neurons that may underlie its ability to abort convulsive activity in persons with epilepsy. |
topic |
hippocampal slices field excitatory postsynaptic potentials adenosine SNE seizure |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2021.610025/full |
work_keys_str_mv |
AT patrickamoateng synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT thomasatagoe synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT thomaskkarikari synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT kennedykekukuia synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT dorcasoseisafo synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT ericwoode synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT brunogfrenguelli synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT samuelbkombian synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus AT samuelbkombian synedrellanodifloraextractdepressesexcitatorysynaptictransmissionandchemicallyinducedinvitroseizuresintherathippocampus |
_version_ |
1724229207010050048 |