AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach.

Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability...

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Bibliographic Details
Main Authors: Hannes M Beyer, Patrick Gonschorek, Sophia L Samodelov, Matthias Meier, Wilfried Weber, Matias D Zurbriggen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4567319?pdf=render