Evaluating cytokine production by flow cytometry using brefeldin A in mice

Summary: Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of interest. To overcome this, we utilize injection of...

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Bibliographic Details
Main Authors: Stephen B. Kovacs, Changhoon Oh, Youssef Aachoui, Edward A. Miao
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302318