GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives

GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives

The ability to reliably express fluorescent reporters or other genes of interest is important for using human pluripotent stem cells (hPSCs) as a platform for investigating cell fates and gene function. We describe a simple expression system, designated GAPTrap (GT), in which reporter genes, includi...

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Bibliographic Details
Main Authors: Tim Kao, Tanya Labonne, Jonathan C. Niclis, Ritu Chaurasia, Zerina Lokmic, Elizabeth Qian, Freya F. Bruveris, Sara E. Howden, Ali Motazedian, Jacqueline V. Schiesser, Magdaline Costa, Koula Sourris, Elizabeth Ng, David Anderson, Antonietta Giudice, Peter Farlie, Michael Cheung, Shireen R. Lamande, Anthony J. Penington, Clare L. Parish, Lachlan H. Thomson, Arash Rafii, David A. Elliott, Andrew G. Elefanty, Edouard G. Stanley
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Stem Cell Reports
Subjects:
human pluripotent stem cells
GAPDH
reporter genes
expression system
lineage tracing
differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671116301394

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http://www.sciencedirect.com/science/article/pii/S2213671116301394

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