Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathy
We have approached the role of cellular stress in neurodegenerative diseases caused by polyglutamine expansion (polyQ) in the context of Spinocerebellar ataxia type 7 (SCA7) that includes retinal degeneration. Using the R7E mouse, in which polyQ-ataxin-7 is specifically over-expressed in rod photore...
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doaj-84a51c9b977f4dfcb3cd189c5200a3f82021-03-20T04:53:54ZengElsevierNeurobiology of Disease1095-953X2007-03-01253571581Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathyKarine Merienne0James Friedman1Masayuki Akimoto2Gretta Abou-Sleymane3Chantal Weber4Anand Swaroop5Yvon Trottier6Department of Molecular Pathology, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/ULP, BP10142, 67404 Illkirch Cédex, CU de Strasbourg, France; Chaire de Génétique Humaine, Collège de France, France; Corresponding authors. Department of Molecular Pathology, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/ULP, BP10142, 67404 Illkirch Cédex, CU de Strasbourg, France. Fax: +33 3 88653246.Departments of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USADepartments of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA; Translational Research Center, Kyoto University Hospital, JapanDepartment of Molecular Pathology, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/ULP, BP10142, 67404 Illkirch Cédex, CU de Strasbourg, France; Chaire de Génétique Humaine, Collège de France, FranceDepartment of Molecular Pathology, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/ULP, BP10142, 67404 Illkirch Cédex, CU de Strasbourg, France; Chaire de Génétique Humaine, Collège de France, FranceDepartments of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA; Department of Human Genetics, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USADepartment of Molecular Pathology, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/ULP, BP10142, 67404 Illkirch Cédex, CU de Strasbourg, France; Chaire de Génétique Humaine, Collège de France, France; Corresponding authors. Department of Molecular Pathology, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/ULP, BP10142, 67404 Illkirch Cédex, CU de Strasbourg, France. Fax: +33 3 88653246.We have approached the role of cellular stress in neurodegenerative diseases caused by polyglutamine expansion (polyQ) in the context of Spinocerebellar ataxia type 7 (SCA7) that includes retinal degeneration. Using the R7E mouse, in which polyQ-ataxin-7 is specifically over-expressed in rod photoreceptors, we previously showed that rod dysfunction correlated to moderate and prolonged activation of the JNK/c-Jun stress pathway. SCA7 retinopathy was also associated with reduced expression of rod-specific genes, including the transcription factor Nrl, which is essential for rod differentiation and function. Here, we report that R7E retinopathy is improved upon breeding with the JunAA knock-in mice, in which JNK-mediated activation of c-Jun is compromised. Expression of Nrl and its downstream targets, which are involved in phototranduction, are partially restored in the JunAA-R7E mice. We further show that c-Jun can directly repress the transcription of Nrl. Our studies suggest that polyQ-induced cellular stress leads to repression of genes necessary for neuronal fate and function.http://www.sciencedirect.com/science/article/pii/S0969996106002920Polyglutamine expansionNeuronal stressJNK/c-Jun pathwayRetinaNrlPhotoreceptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karine Merienne James Friedman Masayuki Akimoto Gretta Abou-Sleymane Chantal Weber Anand Swaroop Yvon Trottier |
spellingShingle |
Karine Merienne James Friedman Masayuki Akimoto Gretta Abou-Sleymane Chantal Weber Anand Swaroop Yvon Trottier Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathy Neurobiology of Disease Polyglutamine expansion Neuronal stress JNK/c-Jun pathway Retina Nrl Photoreceptor |
author_facet |
Karine Merienne James Friedman Masayuki Akimoto Gretta Abou-Sleymane Chantal Weber Anand Swaroop Yvon Trottier |
author_sort |
Karine Merienne |
title |
Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathy |
title_short |
Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathy |
title_full |
Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathy |
title_fullStr |
Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathy |
title_full_unstemmed |
Preventing polyglutamine-induced activation of c-Jun delays neuronal dysfunction in a mouse model of SCA7 retinopathy |
title_sort |
preventing polyglutamine-induced activation of c-jun delays neuronal dysfunction in a mouse model of sca7 retinopathy |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2007-03-01 |
description |
We have approached the role of cellular stress in neurodegenerative diseases caused by polyglutamine expansion (polyQ) in the context of Spinocerebellar ataxia type 7 (SCA7) that includes retinal degeneration. Using the R7E mouse, in which polyQ-ataxin-7 is specifically over-expressed in rod photoreceptors, we previously showed that rod dysfunction correlated to moderate and prolonged activation of the JNK/c-Jun stress pathway. SCA7 retinopathy was also associated with reduced expression of rod-specific genes, including the transcription factor Nrl, which is essential for rod differentiation and function. Here, we report that R7E retinopathy is improved upon breeding with the JunAA knock-in mice, in which JNK-mediated activation of c-Jun is compromised. Expression of Nrl and its downstream targets, which are involved in phototranduction, are partially restored in the JunAA-R7E mice. We further show that c-Jun can directly repress the transcription of Nrl. Our studies suggest that polyQ-induced cellular stress leads to repression of genes necessary for neuronal fate and function. |
topic |
Polyglutamine expansion Neuronal stress JNK/c-Jun pathway Retina Nrl Photoreceptor |
url |
http://www.sciencedirect.com/science/article/pii/S0969996106002920 |
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