Establishment of skin photoaging model and preliminary mechanism of senescence in keratinocytes cell line HaCaT

Objective To establish an efficient skin photoaging model in HaCaT cells and investigate the possible signaling pathways of photoaging of skin. Methods After HaCaT cells were exposed to UVB at 30 mJ/cm2, cell proliferation and cell cycle was detected by CCK-8 assay and flow cytometry. The percentag...

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Bibliographic Details
Main Authors: LIU Chuan, HUANG Xin, WANG Ping, PAN Yun, CAO Di
Format: Article
Language:zho
Published: Editorial Office of Journal of Third Military Medical University 2019-09-01
Series:Di-san junyi daxue xuebao
Subjects:
p53
p21
p16
Online Access:http://aammt.tmmu.edu.cn/Upload/rhtml/201903210.htm