High-Resolution Respirometry for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Fluxes in Permeabilized Cells, Tissue Homogenate and Isolated Mitochondria

High-Resolution Respirometry for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Fluxes in Permeabilized Cells, Tissue Homogenate and Isolated Mitochondria

Whereas mitochondria are well established as the source of ATP in oxidative phosphorylation (OXPHOS), it is debated if they are also the major cellular sources of reactive oxygen species (ROS). Here we describe the novel approach of combining high-resolution respirometry and fluorometric measurement...

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Bibliographic Details
Main Authors: Marina Makrecka-Kuka, Gerhard Krumschnabel, Erich Gnaiger
Format: Article
Language:English
Published: MDPI AG 2015-06-01
Series:Biomolecules
Subjects:
high-resolution respirometry
H2O2 flux
Amplex Red
HEK 293T
mouse brain homogenate
mouse cardiac mitochondria
Online Access:http://www.mdpi.com/2218-273X/5/3/1319

Internet

http://www.mdpi.com/2218-273X/5/3/1319

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