Generation of Rho Zero Cells: Visualization and Quantification of the mtDNA Depletion Process

Generation of Rho Zero Cells: Visualization and Quantification of the mtDNA Depletion Process

Human mitochondrial DNA (mtDNA) is located in discrete DNA-protein complexes, so called nucleoids. These structures can be easily visualized in living cells by utilizing the fluorescent stain PicoGreen®. In contrary, cells devoid of endogenous mitochondrial genomes (ρ0 cells) display no mitochondri...

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Bibliographic Details
Main Authors: Susanna Schubert, Sandra Heller, Birgit Löffler, Ingo Schäfer, Martina Seibel, Gaetano Villani, Peter Seibel
Format: Article
Language:English
Published: MDPI AG 2015-04-01
Series:International Journal of Molecular Sciences
Subjects:
mitochondria
mitochondrial DNA (mtDNA)
nucleoids
ρ0 cells
restriction endonuclease EcoRI
depletion system
Online Access:http://www.mdpi.com/1422-0067/16/5/9850

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http://www.mdpi.com/1422-0067/16/5/9850

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