Generation of Rho Zero Cells: Visualization and Quantification of the mtDNA Depletion Process
Human mitochondrial DNA (mtDNA) is located in discrete DNA-protein complexes, so called nucleoids. These structures can be easily visualized in living cells by utilizing the fluorescent stain PicoGreen®. In contrary, cells devoid of endogenous mitochondrial genomes (ρ0 cells) display no mitochondri...
Main Authors: | Susanna Schubert, Sandra Heller, Birgit Löffler, Ingo Schäfer, Martina Seibel, Gaetano Villani, Peter Seibel |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2015-04-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/16/5/9850 |
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