Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies

Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies

Biologically active human bone morphogenetic protein-4 (hBMP-4) was successfully produced in a prokaryotic host. For this aim, hBMP-4 cDNA was cloned in Escherichia coli (E. coli) and the protein was produced in a non-active aggregated form. After washing and solubilization, in vitro refolding of th...

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Bibliographic Details
Main Authors: Gesa-Maria Gieseler, Kimia Ekramzadeh, Volker Nölle, Svitlana Malysheva, Henning Kempf, Sascha Beutel, Robert Zweigerdt, Ulrich Martin, Ursula Rinas, Thomas Scheper, Iliyana Pepelanova
Format: Article
Language:English
Published: Elsevier 2018-06-01
Series:Biotechnology Reports
Subjects:
Recombinant human bone morphogenetic protein-4 (rhBMP-4)
Inclusion bodies
Refolding
Cation-exchange membrane chromatography
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X18300201

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http://www.sciencedirect.com/science/article/pii/S2215017X18300201

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