Transfection of microRNA mimics should be used with caution

Transfection of microRNA mimics should be used with caution

Transient transfection of chemically synthesized microRNA (miRNA) mimics is being used extensively to study the functions and mechanisms of endogenous miRNAs. However, it remains unclear whether transfected miRNAs behave similarly to endogenous miRNAs. Here we show that transient transfection of miR...

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Bibliographic Details
Main Authors: Hyun Yong eJin, Alicia eGonzalez-Martin, Ana eMiletic, Maoyi eLai, Sarah eKnight, Mohsen eSabouri-Ghomi, Steven R Head, Matthew S Macauley, Robert eRickert, Changchun eXiao
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-12-01
Series:Frontiers in Genetics
Subjects:
miR-155
miR-17~92
Transient transfection
MicroRNA mimics
high molecular weight RNA species
guide strand mutation
Online Access:http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00340/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00340/full

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