The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in...

Full description

Bibliographic Details
Main Authors:	Hansjörg Götzke, Markus Kilisch, Markel Martínez-Carranza, Shama Sograte-Idrissi, Abirami Rajavel, Thomas Schlichthaerle, Niklas Engels, Ralf Jungmann, Pål Stenmark, Felipe Opazo, Steffen Frey
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-09-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-12301-7

id	doaj-852bbbdabda242768df8b322b02e6289
record_format	Article
spelling	doaj-852bbbdabda242768df8b322b02e62892021-05-11T12:01:56ZengNature Publishing GroupNature Communications2041-17232019-09-0110111210.1038/s41467-019-12301-7The ALFA-tag is a highly versatile tool for nanobody-based bioscience applicationsHansjörg Götzke0Markus Kilisch1Markel Martínez-Carranza2Shama Sograte-Idrissi3Abirami Rajavel4Thomas Schlichthaerle5Niklas Engels6Ralf Jungmann7Pål Stenmark8Felipe Opazo9Steffen Frey10NanoTag Biotechnologies GmbHNanoTag Biotechnologies GmbHDepartment of Biochemistry and Biophysics, Stockholm UniversityInstitute of Neuro- and Sensory Physiology, University Medical Center GöttingenNanoTag Biotechnologies GmbHFaculty of Physics and Center for Nanoscience, LMU MunichInstitute of Cellular and Molecular Immunology, University Medical Center GöttingenFaculty of Physics and Center for Nanoscience, LMU MunichDepartment of Biochemistry and Biophysics, Stockholm UniversityNanoTag Biotechnologies GmbHNanoTag Biotechnologies GmbHEpitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.https://doi.org/10.1038/s41467-019-12301-7
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hansjörg Götzke Markus Kilisch Markel Martínez-Carranza Shama Sograte-Idrissi Abirami Rajavel Thomas Schlichthaerle Niklas Engels Ralf Jungmann Pål Stenmark Felipe Opazo Steffen Frey
spellingShingle	Hansjörg Götzke Markus Kilisch Markel Martínez-Carranza Shama Sograte-Idrissi Abirami Rajavel Thomas Schlichthaerle Niklas Engels Ralf Jungmann Pål Stenmark Felipe Opazo Steffen Frey The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications Nature Communications
author_facet	Hansjörg Götzke Markus Kilisch Markel Martínez-Carranza Shama Sograte-Idrissi Abirami Rajavel Thomas Schlichthaerle Niklas Engels Ralf Jungmann Pål Stenmark Felipe Opazo Steffen Frey
author_sort	Hansjörg Götzke
title	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_short	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_full	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_fullStr	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_full_unstemmed	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_sort	alfa-tag is a highly versatile tool for nanobody-based bioscience applications
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-09-01
description	Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.
url	https://doi.org/10.1038/s41467-019-12301-7
work_keys_str_mv	AT hansjorggotzke thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT markuskilisch thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT markelmartinezcarranza thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT shamasograteidrissi thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT abiramirajavel thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT thomasschlichthaerle thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT niklasengels thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT ralfjungmann thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT palstenmark thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT felipeopazo thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT steffenfrey thealfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT hansjorggotzke alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT markuskilisch alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT markelmartinezcarranza alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT shamasograteidrissi alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT abiramirajavel alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT thomasschlichthaerle alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT niklasengels alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT ralfjungmann alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT palstenmark alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT felipeopazo alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications AT steffenfrey alfatagisahighlyversatiletoolfornanobodybasedbioscienceapplications
_version_	1721445496355028992

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

Similar Items