The lateral distance between a proton pump and ATP synthase determines the ATP-synthesis rate

Abstract We have investigated the effect of lipid composition on interactions between cytochrome bo 3 and ATP-synthase, and the ATP-synthesis activity driven by proton pumping. The two proteins were labeled by fluorescent probes and co-reconstituted in large (d ≅ 100 nm) or giant (d ≅ 10 µm) unilame...

Full description

Bibliographic Details
Main Authors: Johannes Sjöholm, Jan Bergstrand, Tobias Nilsson, Radek Šachl, Christoph von Ballmoos, Jerker Widengren, Peter Brzezinski
Format: Article
Language:English
Published: Nature Publishing Group 2017-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-02836-4