Using a chimeric respiratory chain and EPR spectroscopy to determine the origin of semiquinone species previously assigned to mitochondrial complex I

Abstract Background For decades, semiquinone intermediates have been suggested to play an essential role in catalysis by one of the most enigmatic proton-pumping enzymes, respiratory complex I, and different mechanisms have been proposed on their basis. However, the difficulty in investigating compl...

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Bibliographic Details
Main Authors: John J. Wright, Justin G. Fedor, Judy Hirst, Maxie M. Roessler
Format: Article
Language:English
Published: BMC 2020-05-01
Series:BMC Biology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12915-020-00768-6