Oropouche virus cases identified in Ecuador using an optimised qRT-PCR informed by metagenomic sequencing.

Oropouche virus cases identified in Ecuador using an optimised qRT-PCR informed by metagenomic sequencing.

Oropouche virus (OROV) is responsible for outbreaks of Oropouche fever in parts of South America. We recently identified and isolated OROV from a febrile Ecuadorian patient, however, a previously published qRT-PCR assay did not detect OROV in the patient sample. A primer mismatch to the Ecuadorian O...

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Bibliographic Details
Main Authors: Emma L Wise, Sully Márquez, Jack Mellors, Verónica Paz, Barry Atkinson, Bernardo Gutierrez, Sonia Zapata, Josefina Coloma, Oliver G Pybus, Simon K Jackson, Gabriel Trueba, Gyorgy Fejer, Christopher H Logue, Steven T Pullan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://doi.org/10.1371/journal.pntd.0007897

Internet

https://doi.org/10.1371/journal.pntd.0007897

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