A data-independent acquisition-based global phosphoproteomics system enables deep profiling

Phosphoproteomics can provide systematic insights into disease-associated cell signaling changes. Here, the authors present a sensitive workflow integrating library-based and direct data-independent acquisition approaches, and a hybrid spectral library resource for in-depth phosphoproteomic profilin...

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Bibliographic Details
Main Authors: Reta Birhanu Kitata, Wai-Kok Choong, Chia-Feng Tsai, Pei-Yi Lin, Bo-Shiun Chen, Yun-Chien Chang, Alexey I. Nesvizhskii, Ting-Yi Sung, Yu-Ju Chen
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-22759-z