Partição da lipase de Burkholderia cepacia em sistema bifásico aquoso PEG 4000/fosfato

This work aimed to show how to purify the lipase from Burkholderia cepacia by PEG 4000/phosphate aqueous two phase system (ATPS). This system is based on the equilibrium of the polyethylene glycol (PEG) and phosphate salts. A factorial design 22 was used to evaluate the effect of pH (6, 7 and 8) and...

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Bibliographic Details
Main Authors: Giovana da Silva Padilha, Ranulfo Monte Alegre, Elias Basile Tambourgi
Format: Article
Language:Portuguese
Published: Universidade Nove de Julho 2010-01-01
Series:Exacta
Online Access:http://www.redalyc.org/articulo.oa?id=81016917008