Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able...

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Bibliographic Details
Main Authors: Iara Rocha Antunes Pereira Bresolin, Igor Tadeu Lazzarotto Bresolin, Edgar Silveira, Elias Basile Tambourgi, Priscila Gava Mazzola
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2013-12-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012&lng=en&tlng=en

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012&lng=en&tlng=en

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