Use of Biotinylated Ubiquitin for Analysis of Rat Brain Mitochondrial Proteome and Interactome

Applicability of in vitro biotinylated ubiquitin for evaluation of endogenous ubiquitin conjugation and analysis of ubiquitin-associated protein-protein interactions has been investigated. Incubation of rat brain mitochondria with biotinylated ubiquitin followed by affinity chromatography on avidin-...

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Bibliographic Details
Main Authors: Marina V. Medvedeva, Arthur T. Kopylov, Victor G. Zgoda, Alexei E. Medvedev, Olga A. Buneeva
Format: Article
Language:English
Published: MDPI AG 2012-09-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/13/9/11593