CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells

CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells

Targeted gene mutation in the mouse is a primary strategy to understand gene function and relation to phenotype. The Knockout Mouse Project (KOMP) had an initial goal to develop a public resource of mouse embryonic stem (ES) cell clones that carry null mutations in all genes. Indeed, many useful nov...

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Bibliographic Details
Main Authors: Kathleen A. Bishop, Anne Harrington, Evguenia Kouranova, Edward J. Weinstein, Clifford J. Rosen, Xiaoxia Cui, Lucy Liaw
Format: Article
Language:English
Published: Oxford University Press 2016-07-01
Series:G3: Genes, Genomes, Genetics
Subjects:
Dock7
CRISPR/Cas9
KOMP ES cells
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.116.030601

Internet

http://g3journal.org/lookup/doi/10.1534/g3.116.030601

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