Definition of a Novel Plasmid-Based Gene Transfection Protocol of Mammalian Skeletal Muscles by Means of In Vivo Electroporation

We describe an original electroporation protocol for in vivo plasmid DNA transfection. The right hind limbs of C57 mice are exposed to a specifically designed train of permeabilizing electric pulses by transcutaneous application of tailored needle electrodes, immediately after the injection of pEGFP...

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Bibliographic Details
Main Authors: Enrico P. Spugnini, Manuel Scimeca, Bruno Amadio, Giancarlo Cortese, Maurizio Fanciulli, Bruno Vincenzi, Antonio De Luca, Alfonso Baldi
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/21/18/6494