A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

<p>Abstract</p> <p>Background</p> <p>Real-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnostics. Although, the cycle-threshold (<it>Ct</it>) meth...

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Bibliographic Details
Main Authors: Stocchi Laura, Rocchi Marco BL, Sisti Davide, Guescini Michele, Stocchi Vilberto
Format: Article
Language:English
Published: BMC 2008-07-01
Series:BMC Bioinformatics
Online Access:http://www.biomedcentral.com/1471-2105/9/326