Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis
Farrerol (FA) is a flavanone isolated from the Chinese herbal medicine “Man-shan-hong” (<i>Rhododendron dauricum</i> L.). In the present study, FA decreased the viability of SKOV3 cells in a dose- and time-dependent manner, and it induced G2/M cell cycle arrest and cell apoptosis. Cell c...
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doaj-8793b7243746406795724a9852ea1d5c2021-09-09T13:47:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01229400940010.3390/ijms22179400Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated LipolysisJongbeom Chae0Jin Soo Kim1seok tae Choi2Seul Gi Lee3Oyindamola Vivian Ojulari4Young Jin Kang5Taeg Kyu Kwon6Ju-Ock Nam7Department of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaNational Development Institute of Korean Medicine, 94, Gyeongju-si 38540, KoreaDepartment of Pharmacology, College of Medicine, Yeungnam University, Daegu 42415, KoreaDepartment of Immunology, School of Medicine, Keimyung University, Daegu 42601, KoreaDepartment of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Pharmacology, College of Medicine, Yeungnam University, Daegu 42415, KoreaDepartment of Immunology, School of Medicine, Keimyung University, Daegu 42601, KoreaDepartment of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaFarrerol (FA) is a flavanone isolated from the Chinese herbal medicine “Man-shan-hong” (<i>Rhododendron dauricum</i> L.). In the present study, FA decreased the viability of SKOV3 cells in a dose- and time-dependent manner, and it induced G2/M cell cycle arrest and cell apoptosis. Cell cycle distribution analysis via flow cytometry showed that FA decreased G1 populations and increased G2/M populations in SKOV3 cells. Additionally, Western blotting confirmed an increase in the expression level of proteins involved in the cell cycle, e.g., CDK and cyclins. FA-induced apoptosis in SKOV3 cells was also investigated using a TUNEL assay, and increased expression levels of proapoptotic factors, including Caspase-3 and poly ADP ribose polymerase (PARP), through the Extracellular signal-regulated kinase (ERK)/MAPK pathway were investigated. Proinflammatory cytokines (e.g., IL-6, TNF-α, and IL-1) have been identified as a driver of the pathological mechanisms underlying involuntary weight loss and impaired physical function, i.e., cachexia, during cancer; in the present study, we showed that farrerol attenuates TNF-α-induced lipolysis and increases adipogenic differentiation in 3T3-L1 cells. Thus, farrerol could potentially be used as an anticancer agent or anticachetic drug.https://www.mdpi.com/1422-0067/22/17/9400antitumor effectadipogenesiscachexiacell cycle arrestfarrerolovarian cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jongbeom Chae Jin Soo Kim seok tae Choi Seul Gi Lee Oyindamola Vivian Ojulari Young Jin Kang Taeg Kyu Kwon Ju-Ock Nam |
spellingShingle |
Jongbeom Chae Jin Soo Kim seok tae Choi Seul Gi Lee Oyindamola Vivian Ojulari Young Jin Kang Taeg Kyu Kwon Ju-Ock Nam Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis International Journal of Molecular Sciences antitumor effect adipogenesis cachexia cell cycle arrest farrerol ovarian cancer |
author_facet |
Jongbeom Chae Jin Soo Kim seok tae Choi Seul Gi Lee Oyindamola Vivian Ojulari Young Jin Kang Taeg Kyu Kwon Ju-Ock Nam |
author_sort |
Jongbeom Chae |
title |
Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis |
title_short |
Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis |
title_full |
Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis |
title_fullStr |
Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis |
title_full_unstemmed |
Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis |
title_sort |
farrerol induces cancer cell death via erk activation in skov3 cells and attenuates tnf-α-mediated lipolysis |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-08-01 |
description |
Farrerol (FA) is a flavanone isolated from the Chinese herbal medicine “Man-shan-hong” (<i>Rhododendron dauricum</i> L.). In the present study, FA decreased the viability of SKOV3 cells in a dose- and time-dependent manner, and it induced G2/M cell cycle arrest and cell apoptosis. Cell cycle distribution analysis via flow cytometry showed that FA decreased G1 populations and increased G2/M populations in SKOV3 cells. Additionally, Western blotting confirmed an increase in the expression level of proteins involved in the cell cycle, e.g., CDK and cyclins. FA-induced apoptosis in SKOV3 cells was also investigated using a TUNEL assay, and increased expression levels of proapoptotic factors, including Caspase-3 and poly ADP ribose polymerase (PARP), through the Extracellular signal-regulated kinase (ERK)/MAPK pathway were investigated. Proinflammatory cytokines (e.g., IL-6, TNF-α, and IL-1) have been identified as a driver of the pathological mechanisms underlying involuntary weight loss and impaired physical function, i.e., cachexia, during cancer; in the present study, we showed that farrerol attenuates TNF-α-induced lipolysis and increases adipogenic differentiation in 3T3-L1 cells. Thus, farrerol could potentially be used as an anticancer agent or anticachetic drug. |
topic |
antitumor effect adipogenesis cachexia cell cycle arrest farrerol ovarian cancer |
url |
https://www.mdpi.com/1422-0067/22/17/9400 |
work_keys_str_mv |
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