Further Stabilization of Alcalase Immobilized on Glyoxyl Supports: Amination Plus Modification with Glutaraldehyde

Further Stabilization of Alcalase Immobilized on Glyoxyl Supports: Amination Plus Modification with Glutaraldehyde

Alcalase was immobilized on glyoxyl 4% CL agarose beads. This permitted to have Alcalase preparations with 50% activity retention versus Boc-<span style="font-variant: small-caps;">l</span>-alanine 4-nitrophenyl ester. However, the recovered activity versus casein was under 20%...

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Bibliographic Details
Main Authors: Fouzia Hussain, Sara Arana-Peña, Roberto Morellon-Sterling, Oveimar Barbosa, Sabrina Ait Braham, Shagufta Kamal, Roberto Fernandez-Lafuente
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:Molecules
Subjects:
enzyme immobilization
enzyme stabilization
solid phase chemical modification
enzyme amination
glutaraldehyde
crosslinking
Online Access:https://www.mdpi.com/1420-3049/23/12/3188

Internet

https://www.mdpi.com/1420-3049/23/12/3188

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