Targeted insertion of large DNA sequences by homology‐directed repair or non‐homologous end joining in engineered tobacco BY‐2 cells using designed zinc finger nucleases

Targeted insertion of large DNA sequences by homology‐directed repair or non‐homologous end joining in engineered tobacco BY‐2 cells using designed zinc finger nucleases

Abstract Targeted integration of recombinant DNA fragments into plant genomes by DNA double‐strand break (DSB) repair mechanisms has become a powerful tool for precision engineering of crops. However, many targeting platforms require the screening of many transgenic events to identify a low number o...

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Bibliographic Details
Main Authors: Andreas Schiermeyer, Katja Schneider, Janina Kirchhoff, Thomas Schmelter, Natalie Koch, Ke Jiang, Denise Herwartz, Ryan Blue, Pradeep Marri, Pon Samuel, David R. Corbin, Steven R. Webb, Delkin O. Gonzalez, Otto Folkerts, Rainer Fischer, Helga Schinkel, W. Michael Ainley, Stefan Schillberg
Format: Article
Language:English
Published: Wiley 2019-07-01
Series:Plant Direct
Subjects:
DNA recombination
electroporation
gene targeting
genome editing
particle bombardment
split marker genes
Online Access:https://doi.org/10.1002/pld3.153

Internet

https://doi.org/10.1002/pld3.153

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