An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species

An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species

Abstract Vector-free allele exchange (VFAE) is a newly developed protocol for genome editing in Pseudomonas species. Although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones still appear on the plat...

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Bibliographic Details
Main Authors: Ahmed E. Gomaa, Chen Zhang, Zhimin Yang, Liguo Shang, Shijie Jiang, Zhiping Deng, Yuhua Zhan, Wei Lu, Min Lin, Yongliang Yan
Format: Article
Language:English
Published: SpringerOpen 2017-06-01
Series:AMB Express
Subjects:
Vector-free allele exchange (VFAE)
Homologous recombination
Pseudomonas stutzeri
Escherichia coli
Bacillus subtilis
Genome editing
Online Access:http://link.springer.com/article/10.1186/s13568-017-0425-y

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http://link.springer.com/article/10.1186/s13568-017-0425-y

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