An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species
Abstract Vector-free allele exchange (VFAE) is a newly developed protocol for genome editing in Pseudomonas species. Although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones still appear on the plat...
Main Authors: | Ahmed E. Gomaa, Chen Zhang, Zhimin Yang, Liguo Shang, Shijie Jiang, Zhiping Deng, Yuhua Zhan, Wei Lu, Min Lin, Yongliang Yan |
---|---|
Format: | Article |
Language: | English |
Published: |
SpringerOpen
2017-06-01
|
Series: | AMB Express |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13568-017-0425-y |
Similar Items
-
NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli
by: Guihua Hu, et al.
Published: (2019-09-01) -
Study of bacterial interactions in reconstituted hydrocarbon-degrading bacterial consortia from a local collection, for the bioremediation of weathered oily-soils
by: Shaikha Y. Alsayegh, et al.
Published: (2021-03-01) -
Optimization and characterization of Alginic acid synthesized from a novel strain of Pseudomonas stutzeri
by: Meena S, et al.
Published: (2020-09-01) -
A Case of Pseudomonas stutzeri Bacteremia in a Patient with Hematologic Malignancy
by: Ahmet ÖZBEK, et al.
Published: (2010-03-01) -
RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME
by: Purkan Purkan, et al.
Published: (2016-11-01)