Generation of an artificial double promoter for protein expression in Bacillus subtilis through a promoter trap system.

Bacillus subtilis is an attractive host for production of recombinant proteins. Promoters and expression plasmid backbones have direct impacts on the efficiency of gene expression. To screen and isolate strong promoters, a promoter trap vector pShuttleF was developed in this study. Using the vector,...

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Bibliographic Details
Main Authors: Mingming Yang, Weiwei Zhang, Shengyue Ji, Pinghua Cao, Yulin Chen, Xin Zhao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3568030?pdf=render