Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat Diet
The peroxisome proliferator-activated receptor-γ coactivators (PGC-1) are transcriptional coactivators with an important role in mitochondrial biogenesis and regulation of genes involved in the electron transport chain and oxidative phosphorylation in oxidative tissues including cardiac tissue. Thes...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-06-01
|
Series: | Metabolites |
Subjects: | |
Online Access: | http://www.mdpi.com/2218-1989/2/2/366 |
id |
doaj-886c066bff0f47668f9e95b24f98f337 |
---|---|
record_format |
Article |
spelling |
doaj-886c066bff0f47668f9e95b24f98f3372020-11-24T20:54:39ZengMDPI AGMetabolites2218-19892012-06-012236638110.3390/metabo2020366Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat DietGregor McCombieGema Medina-GomezChristopher J LelliottAntonio Vidal-PuigJulian L GriffinThe peroxisome proliferator-activated receptor-γ coactivators (PGC-1) are transcriptional coactivators with an important role in mitochondrial biogenesis and regulation of genes involved in the electron transport chain and oxidative phosphorylation in oxidative tissues including cardiac tissue. These coactivators are thought to play a key role in the development of obesity, type 2 diabetes and the metabolic syndrome. In this study we have used a combined metabolomic and lipidomic analysis of cardiac tissue from the PGC-1β null mouse to examine the effects of a high fat diet on this organ. Multivariate statistics readily separated tissue from PGC-1β null mice from their wild type controls either in gender specific models or in combined datasets. This was associated with an increase in creatine and a decrease in taurine in the null mouse, and an increase in myristic acid and a reduction in long chain polyunsaturated fatty acids for both genders. The most profound changes were detected by liquid chromatography mass spectrometry analysis of intact lipids with the tissue from the null mouse having a profound increase in a number of triglycerides. The metabolomic and lipodomic changes indicate PGC-1β has a profound influence on cardiac metabolism.http://www.mdpi.com/2218-1989/2/2/366metabolic syndromeobesityperoxisome proliferator activated receptorsperoxisome proliferator-activated receptor-γ coactivator 1-βfunctional genomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gregor McCombie Gema Medina-Gomez Christopher J Lelliott Antonio Vidal-Puig Julian L Griffin |
spellingShingle |
Gregor McCombie Gema Medina-Gomez Christopher J Lelliott Antonio Vidal-Puig Julian L Griffin Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat Diet Metabolites metabolic syndrome obesity peroxisome proliferator activated receptors peroxisome proliferator-activated receptor-γ coactivator 1-β functional genomics |
author_facet |
Gregor McCombie Gema Medina-Gomez Christopher J Lelliott Antonio Vidal-Puig Julian L Griffin |
author_sort |
Gregor McCombie |
title |
Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat Diet |
title_short |
Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat Diet |
title_full |
Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat Diet |
title_fullStr |
Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat Diet |
title_full_unstemmed |
Metabolomic and Lipidomic Analysis of the Heart of Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-β Knock Out Mice on a High Fat Diet |
title_sort |
metabolomic and lipidomic analysis of the heart of peroxisome proliferator-activated receptor-γ coactivator 1-β knock out mice on a high fat diet |
publisher |
MDPI AG |
series |
Metabolites |
issn |
2218-1989 |
publishDate |
2012-06-01 |
description |
The peroxisome proliferator-activated receptor-γ coactivators (PGC-1) are transcriptional coactivators with an important role in mitochondrial biogenesis and regulation of genes involved in the electron transport chain and oxidative phosphorylation in oxidative tissues including cardiac tissue. These coactivators are thought to play a key role in the development of obesity, type 2 diabetes and the metabolic syndrome. In this study we have used a combined metabolomic and lipidomic analysis of cardiac tissue from the PGC-1β null mouse to examine the effects of a high fat diet on this organ. Multivariate statistics readily separated tissue from PGC-1β null mice from their wild type controls either in gender specific models or in combined datasets. This was associated with an increase in creatine and a decrease in taurine in the null mouse, and an increase in myristic acid and a reduction in long chain polyunsaturated fatty acids for both genders. The most profound changes were detected by liquid chromatography mass spectrometry analysis of intact lipids with the tissue from the null mouse having a profound increase in a number of triglycerides. The metabolomic and lipodomic changes indicate PGC-1β has a profound influence on cardiac metabolism. |
topic |
metabolic syndrome obesity peroxisome proliferator activated receptors peroxisome proliferator-activated receptor-γ coactivator 1-β functional genomics |
url |
http://www.mdpi.com/2218-1989/2/2/366 |
work_keys_str_mv |
AT gregormccombie metabolomicandlipidomicanalysisoftheheartofperoxisomeproliferatoractivatedreceptorgcoactivator1bknockoutmiceonahighfatdiet AT gemamedinagomez metabolomicandlipidomicanalysisoftheheartofperoxisomeproliferatoractivatedreceptorgcoactivator1bknockoutmiceonahighfatdiet AT christopherjlelliott metabolomicandlipidomicanalysisoftheheartofperoxisomeproliferatoractivatedreceptorgcoactivator1bknockoutmiceonahighfatdiet AT antoniovidalpuig metabolomicandlipidomicanalysisoftheheartofperoxisomeproliferatoractivatedreceptorgcoactivator1bknockoutmiceonahighfatdiet AT julianlgriffin metabolomicandlipidomicanalysisoftheheartofperoxisomeproliferatoractivatedreceptorgcoactivator1bknockoutmiceonahighfatdiet |
_version_ |
1716793814021046272 |